NASC Stock Detail Page
NASC ID: N849938
Name: WiscDsLox257H08
ABRC stock number: CS849938
Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line. Associated Polymorphisms: Wiscseq_DsLox257H08.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT2G24140.1 (chr 2 pos 10264243 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank ID: KG796971. Wiscseq_DsLox257H08.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G20410.1-UTR3 and AT2G20420.1-P355 (chr 2 pos 8805081 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG796970. Wiscseq_DsLox257H08.3 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G22120.1 (chr 4 pos 11717298 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG796972. Wiscseq_DsLox257H08.4 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G64510.1 (chr 5 pos 25784922 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG796973.
Donation Date: 2004-12-01
| Donated by: | University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman |
Donor Number: WiscDsLox257H08
Part of : Wisconsin DsLox T-DNA lines
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available to order at NASC | £11.00 |
Germplasm Info
Mutagen: T-DNA Insertion
Background: Col-2
Transformation method: Agrobacterium
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.